The initial velocity for each trial
was plotted against the concentration of lactose. Note that 6
more trials were plotted and that the concentration of lactose
increases by 0.5mM for each trial. The concentration of lactase
remains constant for each trial. From this curve, the maximum
velocity of the reaction, vmax, can be estimated. This is the point at which
the curve reaches a plateau. Vmax may be estimated to be 75 umol/min. The Michaelis-Menten
constant, Km, can also be estimated. This is the point on
the curve that is half way up the curve before it starts to plateau.
Km
may be estimated to be approximately 0.12mM. In order to get
accurate values for vmax and Km, a Lineweaver-Burk plot can be made.
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